TATA元件指导RNA聚合酶II和III进行双向转录。
TATA elements direct bi-directional transcription by RNA polymerases II and III.
作者信息
Huang W, Wong J M, Bateman E
机构信息
Department of Microbiology, University of Vermont, Burlington 05405 USA.
出版信息
Nucleic Acids Res. 1996 Mar 15;24(6):1158-63. doi: 10.1093/nar/24.6.1158.
Eukaryotic promoter elements specify the direction and efficiency of transcription, as well as the type of RNA polymerase to be used. One such element, the TATA box, is thought to participate in determining the direction of transcription and can function within promoters for RNA polymerase II or III, depending on the sequence context. In this report the ability of four different TATA boxes to support transcription in vitro was determined. It was found that TATA elements are not directional. However, they support transcription by RNA polymerases II and III. An upstream activating sequence was found to stimulate downstream transcription by RNA polymerase II and to inhibit upstream transcription by RNA polymerases II and III. Thus a promoter necessarily consists of a TATA element and upstream sequences in order to specify the direction of transcription and the type of polymerase to be used.
真核生物启动子元件决定转录的方向和效率，以及所使用的RNA聚合酶的类型。其中一个这样的元件，即TATA框，被认为参与决定转录方向，并且根据序列背景可在RNA聚合酶II或III的启动子内发挥作用。在本报告中，测定了四种不同TATA框在体外支持转录的能力。发现TATA元件没有方向性。然而，它们支持RNA聚合酶II和III的转录。发现一个上游激活序列可刺激RNA聚合酶II的下游转录，并抑制RNA聚合酶II和III的上游转录。因此，一个启动子必然由一个TATA元件和上游序列组成，以便确定转录方向和所使用的聚合酶类型。
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