Blaiseau P L, Lafay J F
Cryptogamie, Institut de Génétique et Microbiologie, Université Paris Sud, Orsay, France.
Gene. 1992 Oct 21;120(2):243-8. doi: 10.1016/0378-1119(92)90099-b.
Chitinase 1 (Chi1) is the major extracellular chitinase from the hyperparasitic fungus, Aphanocladium album. We determined the complete sequence of the chromosomal and cDNA copies of the structural gene (chi1) coding for Chi1. The coding region is interrupted by three short introns (55, 53 and 49 bp long). Chi1 is 423 aa long and begins with a stretch of 34 aa not found in the mature protein. The Chi1 sequence presents overall similarities with bacterial chitinases from Serratia marcescens and Bacillus circulans. Compared with other chitinases, A. album Chi1 has only two short similarity regions (12 and 8 aa long), which are also found in bacterial, yeast and some plant chitinases.
几丁质酶1（Chi1）是来自超寄生真菌白绒盖牛肝菌的主要细胞外几丁质酶。我们测定了编码Chi1的结构基因（chi1）的染色体和cDNA拷贝的完整序列。编码区被三个短内含子（分别长55、53和49 bp）中断。Chi1长423个氨基酸，起始部分有一段34个氨基酸的序列在成熟蛋白中不存在。Chi1序列与粘质沙雷氏菌和环状芽孢杆菌的细菌几丁质酶总体相似。与其他几丁质酶相比，白绒盖牛肝菌Chi1只有两个短的相似区域（分别长12和8个氨基酸），在细菌、酵母和一些植物几丁质酶中也有发现。
