Terakawa T, Takaya N, Horiuchi H, Koike M, Takagi M
Central Research Laboratories, Hokko Chemical Ind. Co., 2165 Toda, 243, Atsugi-shi, Kanagawa, Japan.
Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, 113, Tokyo, Japan.
Plant Cell Rep. 1997 Apr;16(7):439-443. doi: 10.1007/BF01092762.
We have studied whether a chitinase involved in cell autolysis of a filamentous fungus,Rhizopus oligosporus, can operate as an antifungal defense system in tobacco. Thechi1 gene was introduced into tobacco by theAgrobacterium tumefaciens leaf disc system. Among 22 transgenic tobacco plants, 2 were selected and their individual homozygous progeny, Tch1-1 and Tch2-1, were studied. Chitinase activity in the extracts of young leaves from Tch1-1 or Tch2-1, in which thechi1 gene product was detected by Western blot analysis, was three- to four-fold higher than that from the control plants. A fungal infection assay on the leaves infected with the discomycete pathogensSclerotinia sclerotiorum andBotrytis cinerea revealed that the symptoms observed with these two were remarkably suppressed as compared with the control leaves.
我们研究了一种参与丝状真菌少孢根霉细胞自溶的几丁质酶是否能在烟草中作为一种抗真菌防御系统发挥作用。通过根癌农杆菌叶盘系统将chi1基因导入烟草。在22株转基因烟草植株中，挑选出2株，并对其各自的纯合后代Tch1-1和Tch2-1进行了研究。通过蛋白质免疫印迹分析在Tch1-1或Tch2-1的幼叶提取物中检测到chi1基因产物，其几丁质酶活性比对照植株高3至4倍。对感染盘菌病原体核盘菌和灰葡萄孢的叶片进行真菌感染试验，结果显示，与对照叶片相比，这两种病原体引起的症状得到了显著抑制。
