Zaccaria A, Martinelli G, Buzzi M, Zuffa E, Zamagni M D, Testoni N, Russo D, Guerrasio A, Saglio G, Tura S
Centro di Genetica Oncologica, Università di Bologna, Italy.
Haematologica. 1992 Jan-Feb;77(1):16-20.
Some reports in the recent literature have shown that the site of molecular rearrangement within the M-BCR area may have a prognostic value in Ph1 + CML patients. A number of studies have, however, failed to demonstrate this finding. Here we report the molecular rearrangements of 107 patients and their clinical follow-up.
Localization of the breakpoints was determined according to conventional criteria, after digestion with 2 to 4 restriction endonucleases and hybridization with 1 or 2 molecular probes.
Sixty two patients were rearranged in the 5' area and 45 in the 3' area: there was no difference between the survival curves of the two groups, at least not after 3 years of follow-up.
The site of the breakpoint within the M-BCR has no prognostic significance in our series.
近期文献中的一些报告显示，M-BCR区域内分子重排的位点可能对Ph1+慢性粒细胞白血病患者具有预后价值。然而，许多研究未能证实这一发现。在此，我们报告107例患者的分子重排情况及其临床随访结果。
在用2至4种限制性内切酶消化并与1或2种分子探针杂交后，根据常规标准确定断点的定位。
62例患者在5'区域发生重排，45例在3'区域发生重排：两组的生存曲线之间没有差异，至少在3年的随访后没有差异。
在我们的研究系列中，M-BCR内断点的位点没有预后意义。
