是否存在一种位于质膜的阴离子敏感ATP酶？

Is there a plasma membrane-located anion-sensitive ATPase?

作者信息

van Amelsvoort J M, de Pont J J, Bonting S L

出版信息

Biochim Biophys Acta. 1977 Apr 18;466(2):283-301. doi: 10.1016/0005-2736(77)90225-5.

Abstract

A study of the intracellular localization of HCO-3-stimulated, SCN--inhibited magnesium-dependent ATPase was performed in gill tissue of the rainbow trout (Salmo irideus), rabbit kidney and rabbit gastric mucosa. Tissue homogenates were subjected to centrifugal fractionation, and the microsomal (60 min 100 000 X g) and light mitochondrial (20 min 20 000 X g) fractions were further fractionated by density gradient centrifugation. Subfractions were characterized by marker enzyme assays and electron microscopic observation. In trout gill indications for an exclusively mitochondrial localization were found. In kidney no definite conclusions could be drawn. In rabbit gastric mucosa initially an apparently non-mitochondrial HCO-3-stimulated ATPase, in addition to a mitochondrial one, was found and its characteristics were studied. Further studies showed that this ATPase also appears to be of mitochondrial origin and probably represents mitochondrial inner membranes. Possible explanations for earlier conflicting reports concerning the localization of this enzyme in gastric mucosa and other tissues are discussed.

摘要

在虹鳟（Salmo irideus）鳃组织、兔肾和兔胃黏膜中，对受HCO₃⁻刺激、受SCN⁻抑制的镁依赖性ATP酶的细胞内定位进行了研究。将组织匀浆进行离心分级分离，微粒体（60分钟，100 000×g）和轻线粒体（20分钟，20 000×g）级分再通过密度梯度离心进一步分级分离。通过标记酶测定和电子显微镜观察对亚级分进行表征。在虹鳟鳃中发现该酶仅定位于线粒体。在肾脏中无法得出明确结论。在兔胃黏膜中，最初除了一种线粒体ATP酶外，还发现了一种明显非线粒体的受HCO₃⁻刺激的ATP酶，并对其特性进行了研究。进一步研究表明，这种ATP酶似乎也起源于线粒体，可能代表线粒体内膜。讨论了关于该酶在胃黏膜和其他组织中定位的早期相互矛盾报道的可能解释。