Van Amelsvoort J M, De Pont J J, Stols A L, Bonting S L
Biochim Biophys Acta. 1977 Nov 15;471(1):78-91. doi: 10.1016/0005-2736(77)90395-9.
A study has been made to determine whether renal plasma membranes contain an HCO3 stimulated, ouabain insensitive Mg ATPase. Purified mitochondrial, microsomal and brush border membrane fractions have been isolated from rabbit kidney. The microsomal anion-sensitive ATPase activity appears to be entirely of mitochondrial origin on the basis of the effects of inhibitors of mitochondrial Mg ATPase. The brush border membrane fraction is contaminated with mitochondrial fragments and contains an Mg ATPase activity with low anion-sensitivity. Further purification of this fraction causes parallel decreases in anion-sensitivity of the Mg ATPase activity and in cytochrome c oxidase activity. These results indicate that conclusions previously reached by other investigators for a role of anion-sensitive Mg ATPase in the bicarbonate reabsorption of the proximal tubule may no longer be tenable.
已开展一项研究以确定肾细胞膜是否含有受HCO₃刺激、哇巴因不敏感的Mg ATP酶。已从兔肾中分离出纯化的线粒体、微粒体和刷状缘膜部分。基于线粒体Mg ATP酶抑制剂的作用，微粒体阴离子敏感ATP酶活性似乎完全源自线粒体。刷状缘膜部分被线粒体碎片污染，且含有阴离子敏感性较低的Mg ATP酶活性。该部分的进一步纯化导致Mg ATP酶活性的阴离子敏感性和细胞色素c氧化酶活性平行下降。这些结果表明，其他研究者先前得出的关于阴离子敏感Mg ATP酶在近端小管碳酸氢盐重吸收中作用的结论可能不再成立。
