WEBSTER H D
J Cell Biol. 1962 Feb;12(2):361-83. doi: 10.1083/jcb.12.2.361.
Wallerian degeneration was produced in guinea pig sciatic nerves by a crush injury. At intervals of 2, 12, 24, 36, 48, 72, and 96 hours after the crush, the nerves were fixed in osmium tetroxide, and blocks from the distal, degenerating segment identified topographically prior to embedding in Araldite or Epon. Phase and electron microscopic study of serial cross- and longitudinal sections reveals a striking, localized accumulation of axonal mitochondria which precedes or accompanies the swelling and fragmentation previously reported by others. These focal accumulations of mitochondria are transient and are most frequently observed in the paranodal axoplasm of large myelinated fibers 24 to 36 hours after crush injury, but are also occasionally identified in small myelinated fibers and unmyelinated axons. Migration and proliferation of axonal mitochondria are considered as possible explanations of these observations.
通过挤压损伤在豚鼠坐骨神经中产生华勒氏变性。在挤压后的2、12、24、36、48、72和96小时的间隔时间，将神经用四氧化锇固定，在嵌入Araldite或Epon之前，从远端、正在变性的节段识别出局部区域的组织块。对连续的横切面和纵切面进行的相衬显微镜和电子显微镜研究显示，轴突线粒体有明显的、局部的聚集，这种聚集先于或伴随其他人先前报道的肿胀和断裂。这些线粒体的局灶性聚集是短暂的，最常于挤压损伤后24至36小时在有髓大纤维的结旁轴质中观察到，但偶尔也在有髓小纤维和无髓轴突中发现。轴突线粒体的迁移和增殖被认为是对这些观察结果的可能解释。
