Armstrong M T, Armstrong P B
Department of Zoology, University of California, Davis 95616-8755.
J Cell Sci. 1992 Jun;102 ( Pt 2):373-85. doi: 10.1242/jcs.102.2.373.
The processes responsible for epithelial spreading during wound healing and embryonic morphogenesis were investigated in an organ culture model in which an epithelial tissue (chick embryo pigmented retinal epithelium) spread over the surface of an aggregate of mesenchyme cells (chick embryo cardiac mesenchyme). The heart mesenchyme aggregate is differentiated into a core of stellate cells associated with a fibronectin-poor matrix surrounded by a cortical zone, 2-5 cells in thickness, of flattened cells embedded in a fibronectin-rich extracellular matrix. Envelopment of the mesenchyme aggregate is accompanied by a movement of the cells and the fibronectin-rich extracellular matrix of the cortex over the core tissue in advance of the spreading pigmented retina tissue. Three distinct processes were identified as contributing to epithelial spreading in this system: (1) active migration of the pigmented retinal epithelium; (2) active contraction of the cortical cells of the mesenchyme aggregate to tow the attached epithelial tissue over the mesenchyme aggregate; and (3) ingression of surface-located cells of the mesenchyme aggregate to decrease the exposed surface area by decreasing the number of cells at the surface.
在一个器官培养模型中研究了伤口愈合和胚胎形态发生过程中上皮细胞铺展的相关过程。在该模型中，一种上皮组织（鸡胚色素视网膜上皮）在间充质细胞聚集体（鸡胚心脏间充质）的表面铺展。心脏间充质聚集体分化为星状细胞核心，其与富含纤连蛋白的基质相关，周围是一个厚度为2 - 5个细胞的皮质区，由嵌入富含纤连蛋白的细胞外基质中的扁平细胞组成。间充质聚集体的包被伴随着皮质区细胞和富含纤连蛋白的细胞外基质在色素沉着视网膜组织铺展之前在核心组织上的移动。在该系统中确定了三个不同的过程有助于上皮细胞铺展：（1）色素视网膜上皮的主动迁移；（2）间充质聚集体皮质细胞的主动收缩，以拉动附着的上皮组织在间充质聚集体上移动；（3）间充质聚集体表面定位细胞的内陷，通过减少表面细胞数量来减小暴露表面积。
