Van Belle S J, De Smet M, Monsaert C, Geerts F, Storme G A, Massart D L
Oncologisch Centrum, Vrije Universiteit Brussel, Belgium.
J Chromatogr. 1992 May 8;576(2):351-7. doi: 10.1016/0378-4347(92)80211-8.
A high-performance liquid chromatographic method is described for separating and determining navelbine and possible metabolites in plasma, cell culture medium and MO4 cells. Navelbine is extracted from these fluids by ion-pair extraction with sodium octylsulphate as the counter-ion at pH 3. The system uses a cyano column as the stationary phase and a mobile phase of acetonitrile-0.12 M phosphate buffer (pH 3) (60:40, v/v). Application of the method to a study of the pharmacokinetic behaviour of navelbine in MO4 mouse fibrosarcoma cells is reported.
描述了一种高效液相色谱法，用于分离和测定血浆、细胞培养基和MO4细胞中的诺维本及其可能的代谢产物。在pH 3条件下，以辛基硫酸钠作为反离子，通过离子对萃取从这些液体中提取诺维本。该系统使用氰基柱作为固定相，乙腈-0.12 M磷酸盐缓冲液（pH 3）（60:40，v/v）作为流动相。报道了该方法在研究诺维本在MO4小鼠纤维肉瘤细胞中的药代动力学行为方面的应用。
