用高效液相色谱法测定小鼠组织中的长春花生物碱。

Determination of vinca alkaloids in mouse tissues by high-performance liquid chromatography.

作者信息

Van Belle S J, De Smet M, De Neve W, Monsaert C, Storme G A, Massart D L

机构信息

Cancer Research Unit Oncologisch Centrum Academisch Ziekenhuis, Vrije Universiteit Brussel, Belgium.

出版信息

J Chromatogr. 1992 Jul 24;578(2):223-9. doi: 10.1016/0378-4347(92)80420-u.

DOI:10.1016/0378-4347(92)80420-u

PMID:1400801

Abstract

A high-performance liquid chromatographic method is described for the determination of vinblastine in various normal mouse tissues, such as lung, heart, liver, kidney and muscles, and in implanted MO4 tumours. Vincristine was used as the internal standard. Freshly obtained mouse tissue or tumour tissue was frozen at -20 degrees C and then lyophilized. After lyophilisation, the dry tissues were pulverized and homogeneously mixed, and an aliquot was suspended in 0.1 M hydrochloric acid. The drugs of interest were then isolated from this suspension using ion-pair extraction at pH 3 with octylsulphate as counter-ion. The obtained extracts were analysed on a reversed-phase system with a cyanopropyl stationary phase. The detection limit was 1 ng/l in plasma and 10 ng/g in tissue. The extraction recoveries of vincristine and vinblastine were between 45 and 67%, and there were no interferences from blank components. Preliminary pharmacokinetic data for different mouse tissues and tumour implanted in muscle tissue are presented.

摘要

描述了一种高效液相色谱法，用于测定各种正常小鼠组织（如肺、心脏、肝脏、肾脏和肌肉）以及植入的MO4肿瘤中的长春碱。使用长春新碱作为内标。将新鲜获取的小鼠组织或肿瘤组织在-20℃下冷冻，然后冻干。冻干后，将干燥的组织研磨并均匀混合，取一份样品悬浮于0.1 M盐酸中。然后在pH 3条件下，以辛基硫酸盐作为反离子，通过离子对萃取从该悬浮液中分离出目标药物。所得提取物在具有氰丙基固定相的反相系统上进行分析。血浆中的检测限为1 ng/l，组织中的检测限为10 ng/g。长春新碱和长春碱的萃取回收率在45%至67%之间，空白成分无干扰。给出了不同小鼠组织以及植入肌肉组织中的肿瘤的初步药代动力学数据。