Gaspari F, Taiocchi L, Pochobradsky M G
Laboratory of Clinical Pharmacokinetics, Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
J Chromatogr. 1992 May 20;577(1):123-8. doi: 10.1016/0378-4347(92)80606-q.
A high-performance liquid chromatographic method was developed to measure the concentration of brodimoprim and its metabolite, hydroxybrodimoprim, in small volumes of blood, plasma and urine. The procedure involved a simple extraction step with chloroform, followed by chromatographic separation on a short reversed-phase column deactivated for the analysis of basic compounds. The column effluent was monitored by fluorescence (excitation wavelength 290 nm, emission wavelength 340 nm). The recoveries of both compounds were similar in all three biological fluids, and averaged 84 and 72%, respectively. The detection limit for both compounds reached 5 ng/ml. No endogenous compound interfered in the assay. The linearity of the method and its within- and between-day precision were analytically satisfactory.
开发了一种高效液相色谱法，用于测定少量血液、血浆和尿液中溴莫普明及其代谢物羟基溴莫普明的浓度。该程序包括用氯仿进行简单萃取步骤，然后在用于分析碱性化合物的短反相柱上进行色谱分离。通过荧光监测柱流出物（激发波长290nm，发射波长340nm）。两种化合物在所有三种生物流体中的回收率相似，平均分别为84%和72%。两种化合物的检测限均达到5ng/ml。测定中无内源性化合物干扰。该方法的线性及其日内和日间精密度在分析上令人满意。
