体液中甲氧苄啶、磺胺甲恶唑和N4-乙酰磺胺甲恶唑的高效液相色谱定量分析

High-performance liquid chromatographic quantitation of trimethoprim, sulfamethoxazole, and N4-acetylsulfamethoxazole in body fluids.

作者信息

Weber A, Opheim K E, Siber G R, Ericson J F, Smith A L

出版信息

J Chromatogr. 1983 Dec 9;278(2):337-45. doi: 10.1016/s0378-4347(00)84793-3.

Abstract

We describe a rapid, precise and simple procedure for the quantitative determination of trimethoprim, sulfamethoxazole, and N4-acetylsulfamethoxazole in body fluids by reversed-phase high-performance liquid chromatography. This method utilizes antipyrine as an internal standard with the compounds detected by dual-wavelength monitoring at 225 nm and 254 nm after a single-step extraction. Precision, sensitivity, and accuracy of this assay are within the range of clinical utility; the coefficient of variation is less than or equal to 3%, sensitivity less than 0.5 micrograms/ml for all compounds, and recovery greater than 97%. The short time for performance and small sample size makes the assay ideal for clinical drug monitoring and pharmacokinetic studies.

摘要

我们描述了一种通过反相高效液相色谱法定量测定体液中甲氧苄啶、磺胺甲恶唑和N4-乙酰磺胺甲恶唑的快速、精确且简单的方法。该方法使用安替比林作为内标，经一步萃取后，通过在225nm和254nm处的双波长监测来检测这些化合物。该测定法的精密度、灵敏度和准确度均在临床应用范围内；变异系数小于或等于3%，所有化合物的灵敏度均小于0.5微克/毫升，回收率大于97%。分析所需时间短且样本量小，使得该测定法非常适合临床药物监测和药代动力学研究。