Saunders P P, Alvarez E, Kantarjian H M
Department of Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
J Chromatogr. 1992 May 20;577(1):37-41. doi: 10.1016/0378-4347(92)80596-i.
A high-performance liquid chromatographic assay for cellular nicotinamide-adenine dinucleotide and thiazole-4-carboxamide-adenine dinucleotide is presented that is appropriate for analysis of these dinucleotides in extracts of Ficoll-purified human leukemic cells. The separation, which is effected by reversed-phase chromatography, is highly reproducible and the limit of quantitation is as low as 10-15 pmol. The stability of these compounds in neutralized perchloric acid extracts is addressed and the applicability of the procedure to clinical specimens is demonstrated.
