用高效液相色谱法准确测量烟酰胺腺嘌呤二核苷酸（NAD⁺）。
Accurate measurement of nicotinamide adenine dinucleotide (NAD⁺) with high-performance liquid chromatography.
作者信息
Yoshino Jun, Imai Shin-Ichiro
机构信息
Center for Human Nutrition, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
出版信息
Methods Mol Biol. 2013;1077:203-15. doi: 10.1007/978-1-62703-637-5_14.
Abstract
Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD(+) levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD(+) measurement, which enables us to analyze various pathophysiological changes in NAD(+) levels in vitro and in vivo.
摘要
烟酰胺腺嘌呤二核苷酸（NAD(+)）在调节包括哺乳动物的代谢、炎症、癌症和衰老等众多生物学和生理途径中起着关键作用。在此，我们描述了一种采用反相高效液相色谱法（HPLC）测定细胞和组织中NAD(+)水平的高度定量方法。该方法提供了准确、可靠且可重复的NAD(+)测量结果，使我们能够在体外和体内分析NAD(+)水平的各种病理生理变化。
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