Otsuka M, Ohmori S
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
J Chromatogr. 1992 Jun 10;577(2):215-20. doi: 10.1016/0378-4347(92)80242-i.
Acetoin was quantitatively oxidized into diacetyl by Fe3+ in 1 M perchloric acid. The reaction of diacetyl with 4,5-dichloro-1,2-diaminobenzene afforded 6,7-dichloro-2,3-dimethylquinoxaline (DCDMQ), which was extracted by benzene containing aldrin (25 ng/ml) as an internal standard, and determined by gas chromatography with electron-capture detection. The method is very simple and sensitive. The detection limit of DCDMQ (either diacetyl or acetoin) was 10 fmol/microliters of the benzene extract, and the determination limit of DCDMQ (either diacetyl or acetoin) was 50 fmol/microliters of the extract. Both acetoin and diacetyl could be determined in 0.1 ml of normal human urine or blood, and both were found in rat liver, kidney and brain. The method was also applied to the determination of acetoin and diacetyl in alcoholic drinks.
在1M高氯酸中，乙偶姻被Fe3+定量氧化为双乙酰。双乙酰与4,5-二氯-1,2-二氨基苯反应生成6,7-二氯-2,3-二甲基喹喔啉（DCDMQ），用含有艾氏剂（25 ng/ml）作为内标的苯进行萃取，并用电子捕获检测气相色谱法进行测定。该方法非常简单且灵敏。DCDMQ（双乙酰或乙偶姻）的检测限为苯萃取液10 fmol/微升，DCDMQ（双乙酰或乙偶姻）的测定限为萃取液50 fmol/微升。乙偶姻和双乙酰均可在0.1 ml正常人尿液或血液中测定，且在大鼠肝脏、肾脏和大脑中均有发现。该方法也应用于酒精饮料中乙偶姻和双乙酰的测定。
