Leis H J, Ozand P T, al Odaib A, Gleispach H
Department of Mass Spectrometry, University Children's Hospital, University of Graz, Austria.
J Chromatogr. 1992 Jul 1;578(1):116-9. doi: 10.1016/0378-4347(92)80232-f.
A highly specific and sensitive assay for N-acetyl-L-aspartic acid has been developed. The trideuterated compound was synthesized and used as an internal standard for gas chromatography with negative-ion chemical ionization mass spectrometry. Urine samples were acidified and extracted with ethyl acetate, and the compounds converted into their pentafluorobenzyl ester derivatives. Under these conditions, sub-picogram amounts of the pure derivatives could be detected. Thus, only microliter volumes of urine samples have to be processed to achieve reliable quantification of "basal" levels of N-acetyl-L-aspartic acid.
已开发出一种用于检测 N-乙酰-L-天冬氨酸的高特异性和高灵敏度检测方法。合成了三重氘代化合物，并将其用作负离子化学电离质谱气相色谱法的内标。尿液样本经酸化后用乙酸乙酯萃取，化合物转化为其五氟苄酯衍生物。在这些条件下，可以检测到亚皮克级量的纯衍生物。因此，只需处理微升体积的尿液样本，就能可靠地定量 N-乙酰-L-天冬氨酸的“基础”水平。
