Jansen E H, van Ginkel L A, van den Berg R H, Stephany R W
National Institute of Public Health and Environmental Protection, Bilthoven, Netherlands.
J Chromatogr. 1992 Sep 16;580(1-2):111-24. doi: 10.1016/0378-4347(92)80531-t.
The role of high-performance liquid chromatography (HPLC) in methods of analysis for anabolic compounds in biological samples is reviewed. Special attention is given to both the separation and detection of anabolic compounds. A distinction is made between on-line detection systems, such as ultraviolet detection and diode-array detection, and off-line detection methods with special emphasis on immunochemical detection methods using non-isotopic labels. A number of applications are given to elucidate the possibilities of HPLC in the analysis of anabolic compounds.
综述了高效液相色谱（HPLC）在生物样品中合成代谢化合物分析方法中的作用。特别关注了合成代谢化合物的分离和检测。区分了在线检测系统，如紫外检测和二极管阵列检测，以及离线检测方法，特别强调了使用非同位素标记的免疫化学检测方法。给出了一些应用实例以阐明HPLC在合成代谢化合物分析中的可能性。
