Sasaki T, Sasaki Y, Kita M, Suzuki K, Watanabe H, Honda M
Department of General Biologics Control, National Institute of Health, Tokyo, Japan.
J Clin Microbiol. 1992 Sep;30(9):2435-40. doi: 10.1128/jcm.30.9.2435-2440.1992.
Mycoplasma fermentans incognitus has attracted much interest either as a cofactor for the progression of AIDS or as a pathogenic agent in non-AIDS-related diseases (S.-C. Lo, M. S. Dawson, P. B. Newton III, M. A. Sonoda, J. W.-K. Shih, W. F. Engler, R. Y.-H. Wang, and D. J. Wear, Am. J. Trop. Med. Hyg. 41:364-376, 1989; S.-C. Lo, M. S. Dawson, M. Wong, P. B. Newton III, M. A. Sonoda, W. F. Engler, R.Y.-H Wang, J. W.-K. Shih, H. J. Alter, and D. J. Wear, Am. J. Trop. Med. Hyg. 41:601-616, 1989; S.-C. Lo, J.W.-K. Shih, N.-Y. Yang, C.-Y. Ou, and R. Y.-H. Wang, Am. J. Trop. Med. Hyg. 40:213-226, 1989). In the present study, the genetic and serologic properties of the incognitus strain and other M. fermentans strains were compared. Furthermore, the replication of human immunodeficiency virus type 1 (HIV-1), determined by reverse transcriptase activity and HIV-1 p24 antigen level, in peripheral blood mononuclear cells was evaluated after stimulation with mycoplasma cell lysates. The psb-2.2 viruslike infectious agent DNA probe, used to identify the incognitus strain in the tissues of AIDS and non-AIDS patients by Lo et al. (Am. J. Trop. Med. Hyg. 41:364-376 and 40:213-226, 1989), showed reaction patterns similar to those of two M. fermentans strains isolated from cell cultures but not to that of the type strain PG18. Restriction enzyme patterns of the incognitus strain with EcoRI and HindIII were also similar to those of M. fermentans strains isolated from cell cultures. There were no remarkable differences in the immunoblot profiles between the incognitus strain and the other M. fermentans strains. These results suggest that the incognitus strain is not a unique strain among M. fermentans strains. Further, cell lysates of each M. fermentans strain could also enhance the replication of HIV-1 to a level similar to that of the incognitus strain as determined by the reverse transcriptase activity and the amount of the p24 antigen.
发酵支原体隐匿株（Mycoplasma fermentans incognitus）作为艾滋病进展的辅助因子或非艾滋病相关疾病的病原体已引起了广泛关注（S.-C. 罗、M.S. 道森、P.B. 牛顿三世、M.A. 园田、J.W.-K. 施、W.F. 恩格勒、R.Y.-H. 王和D.J. 韦尔，《美国热带医学与卫生杂志》41:364 - 376，1989；S.-C. 罗、M.S. 道森、M. 王、P.B. 牛顿三世、M.A. 园田、W.F. 恩格勒、R.Y.-H. 王、J.W.-K. 施、H.J. 阿尔特和D.J. 韦尔，《美国热带医学与卫生杂志》41:601 - 616，1989；S.-C. 罗、J.W.-K. 施、N.-Y. 杨、C.-Y. 欧和R.Y.-H. 王，《美国热带医学与卫生杂志》40:213 - 226，1989）。在本研究中，比较了隐匿株与其他发酵支原体菌株的遗传和血清学特性。此外，在用支原体细胞裂解物刺激后，通过逆转录酶活性和HIV - 1 p24抗原水平测定了1型人类免疫缺陷病毒（HIV - 1）在外周血单核细胞中的复制情况。Lo等人（《美国热带医学与卫生杂志》41:364 - 376和40:213 - 226，1989）用于在艾滋病和非艾滋病患者组织中鉴定隐匿株的psb - 2.2病毒样感染因子DNA探针，显示出与从细胞培养物中分离的两株发酵支原体菌株相似的反应模式，但与模式菌株PG18的反应模式不同。隐匿株用EcoRI和HindIII酶切后的图谱也与从细胞培养物中分离的发酵支原体菌株相似。隐匿株与其他发酵支原体菌株之间的免疫印迹图谱没有显著差异。这些结果表明，隐匿株在发酵支原体菌株中并非独特菌株。此外，通过逆转录酶活性和p24抗原量测定，各发酵支原体菌株的细胞裂解物也能将HIV - 1的复制增强到与隐匿株相似的水平。
