Larsen I B, Freund M, Munksgaard E C
Department of Dental Materials, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
J Dent Res. 1992 Nov;71(11):1851-3. doi: 10.1177/00220345920710111701.
The surface microhardness of specimens made of a BisGMA/TEGDMA polymer was measured before and during treatment for 60 days with phosphate buffer or phosphate-buffered esterase solution with an activity corresponding to the mean hydrolase activity of human saliva. The hardness of the buffer-treated specimens was unchanged during the incubation period, while that of the esterase-treated specimens decreased gradually. After about five days of treatment, no further change in hardness was observed for up to 60 days. Based on the difference in the calculated hardness of the specimens as a function of the applied load during measurement, it was estimated that the mean microhardness of the outermost surface layer of the esterase-treated specimens was diminished by about 15%, compared with that of the buffer-treated specimens. From these results, it was concluded that the wear resistance of a BisGMA/TEGDMA polymer is most likely diminished by hydrolases in saliva.
用磷酸缓冲液或具有与人唾液平均水解酶活性相当的活性的磷酸缓冲酯酶溶液对由双甲基丙烯酸缩水甘油酯/三乙二醇二甲基丙烯酸酯（BisGMA/TEGDMA）聚合物制成的试样进行60天处理，在此之前及处理过程中测量其表面显微硬度。经缓冲液处理的试样在孵育期内硬度不变，而经酯酶处理的试样硬度逐渐降低。处理约五天后，在长达60天的时间内未观察到硬度有进一步变化。根据测量过程中试样计算硬度随施加负荷的变化差异，估计与经缓冲液处理的试样相比，经酯酶处理的试样最外层表面层的平均显微硬度降低了约15%。从这些结果得出结论，唾液中的水解酶很可能会降低BisGMA/TEGDMA聚合物的耐磨性。
