BUCKLEY I K
J Cell Biol. 1962 Sep;14(3):401-20. doi: 10.1083/jcb.14.3.401.
Unfixed, compressed acinar cells of rat pancreas, isolated by mechanical and enzymatic means, were examined by phase microscopy and photomicrographed using 35 mm film and electronic flash illumination. Similarly, observations were made on Walker carcinoma cells; in addition, these cells were treated with solutions containing either phosphatidase A or enzyme inhibitors. Acinar cells contained, besides nuclei, perinuclear droplets and secretion granules, various membranous and vacuolar structures. The basal cytoplasm showed parallel dark lines interpreted as endoplasmic reticulum. In some cells, fragmentation of the reticulum was followed by the direct incorporation of fragments into simple myelin figures. In other cells it appeared that phase-lucent linear structures and vacuoles were derived by dilatation of cisternae of the endoplasmic reticulum. Perinuclear fluid collections arose either by dilation of the perinuclear cisternae of the endoplasmic reticulum or by fluid dilatation of the nuclear envelope. Phosphatidase A disrupted early vacuoles of Walker carcinoma cells. From this and the direct involvement of elements of the endoplasmic reticulum in myelin figures, it was concluded that the membranes limiting the endoplasmic reticulum incorporate phosphatides in continuous layers. While many severely injured cells formed large vacuoles, others developed concentrically laminated myelin figures; it was concluded that both types of structure derived from phosphatides liberated intracellularly, the vacuoles by vesicular myelin figure formation.
通过机械和酶法分离的大鼠胰腺未固定的压缩腺泡细胞，用相差显微镜检查并用35毫米胶片和电子闪光照明进行显微摄影。同样，对沃克癌细胞进行了观察；此外，用含有磷脂酶A或酶抑制剂的溶液处理这些细胞。腺泡细胞除了含有细胞核、核周小滴和分泌颗粒外，还含有各种膜性和液泡状结构。基底细胞质显示出平行的暗线，被解释为内质网。在一些细胞中，内质网碎片化后，碎片直接整合到简单的髓鞘样结构中。在其他细胞中，似乎透明的线性结构和液泡是由内质网池的扩张形成的。核周液体积聚要么是由于内质网的核周池扩张，要么是由于核膜的液体扩张。磷脂酶A破坏了沃克癌细胞的早期液泡。由此以及内质网成分直接参与髓鞘样结构，得出结论：限制内质网的膜以连续层的形式整合磷脂。虽然许多严重受损的细胞形成了大液泡，但其他细胞则形成了同心层状的髓鞘样结构；得出结论，这两种结构都来源于细胞内释放的磷脂，液泡是通过囊泡状髓鞘样结构形成的。
