髓鞘样结构的电子显微镜研究。
An electron microscope study of myelin figures.
作者信息
STOECKENIUS W
出版信息
J Biophys Biochem Cytol. 1959 May 25;5(3):491-500. doi: 10.1083/jcb.5.3.491.
Abstract
In the electron microscope, thin sections of OsO(4)-fixed myelin figures from the phospholipide fraction of human brain show a pattern of parallel dark lines with a repeating period of about 40 A. It is shown that the dark lines probably represent the reaction product of OsO(4) with double bonds in the fatty acid chains, thereby marking the central portion of one bimolecular lamella. The addition of globin results in dense lines 25 to 50 A wide that cover the surface of the myelin figures. When such a figure consists of only two bimolecular leaflets of lipide covered with globin, the structure shows striking similarity to the image of cell membranes in fixed tissue sections. A hypothetical schema is given of the molecular structure of the figure, and the distribution of OsO(4) in it.
摘要
在电子显微镜下，用四氧化锇固定的人脑磷脂部分的髓鞘样结构薄片显示出平行暗线的图案，重复周期约为40埃。结果表明，暗线可能代表四氧化锇与脂肪酸链中双键的反应产物，从而标记了一个双分子层的中心部分。加入球蛋白会产生25至50埃宽的致密线，覆盖髓鞘样结构的表面。当这样的结构仅由覆盖有球蛋白的两个脂质双分子小叶组成时，其结构与固定组织切片中细胞膜的图像惊人地相似。给出了该结构的分子结构及其中四氧化锇分布的假设示意图。
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