Dietrich C P, McDuffie N M, Sampaio L O
J Chromatogr. 1977 Jan 11;130:299-304. doi: 10.1016/s0021-9673(00)89809-x.
A micro-method for the identification of most acidic mucopolysaccharides by agarose gel electrophoresis with three different buffer systems is described. In barbital buffer the mucopolysaccharides are fractionated from each other as a function of their net charge, whereas in a diamine buffer the fractionation is probably achieved according to the degree to which they are bound to the diamine. A combination of barbital and diaminopropane buffers in two-dimensional electrophoresis for the identification of mucopolysaccharides is also described.
本文描述了一种通过琼脂糖凝胶电泳，利用三种不同缓冲系统鉴定大多数酸性粘多糖的微量方法。在巴比妥缓冲液中，粘多糖根据其净电荷相互分离，而在二胺缓冲液中，分离可能是根据它们与二胺结合的程度实现的。还描述了在二维电泳中使用巴比妥和二氨基丙烷缓冲液组合来鉴定粘多糖的方法。
