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Lymphocyte immunophenotyping by flow cytometry in normal adults. Comparison of fresh whole blood lysis technique, Ficoll-Paque separation and cryopreservation.

作者信息

Romeu M A, Mestre M, González L, Valls A, Verdaguer J, Corominas M, Bas J, Massip E, Buendia E

机构信息

Servei d'Immunologia, Hospital Duran i Reynals, Barcelona, Catalunya, Spain.

出版信息

J Immunol Methods. 1992 Sep 18;154(1):7-10. doi: 10.1016/0022-1759(92)90206-9.

DOI:10.1016/0022-1759(92)90206-9
PMID:1401946
Abstract

In the present report we have assessed the extent to which Ficoll-Paque separation and cryopreservation of mononuclear cells alter the measurement of lymphocyte subsets by flow cytometry. Standard Ficoll-Paque separation increased the percentage of CD4+, CD19+ and CD4+CD45RA+ cells, as well as decreasing that of CD8+, and CD4+CD29+ cells, compared to the fresh whole blood lysis technique. Moreover, cryopreservation caused a depletion of CD4+ p80+ cells, but normal whole blood values were restored following a short incubation.

摘要

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