Motley D, Meyer M P, King R A, Naus G J
University of Pittsburgh, School of Medicine, Department of Pathology, Pennsylvania, USA.
Am J Clin Pathol. 1996 Jan;105(1):38-43. doi: 10.1093/ajcp/105.1.38.
Normal reference values for flow cytometric immunophenotypic lymphocyte markers for cord blood (CB) were determined using sufficient numbers of subjects for clinical laboratory use. Samples from 202 normal gestations were processed by whole blood lysis and analyzed in the following combinations: CD14/45, CD4/3, CD8/3, CD45RA/4, CD29/4, CD56/3, Cd19/3, CD19/10. Thirty-five adult laboratory volunteers were analyzed as controls. When compared to adults, CB showed increased relative percentages of naive T-helper cells, B cells, immature B cells, and CD8+3-cells and decreased T cells, cytotoxic T cells, activated T-helper cells, and large granular lymphocytes (CD56+3+). Significant differences were also found when CB samples were stratified by sex and race. These results provide clinical laboratory normal reference values for lymphocyte markers for CB, demonstrate the need for determining separate standard reference values for significantly different patient populations, provide the basis for future investigation of pathologic gestations and for clinical laboratory applications, and provide insight into early immunologic development.
使用足够数量的受试者来确定用于临床实验室的脐带血（CB）流式细胞术免疫表型淋巴细胞标志物的正常参考值。对来自202例正常妊娠的样本进行全血裂解处理，并以下列组合进行分析：CD14/45、CD4/3、CD8/3、CD45RA/4、CD29/4、CD56/3、Cd19/3、CD19/10。对35名成年实验室志愿者进行分析作为对照。与成年人相比，CB显示出初始T辅助细胞、B细胞、未成熟B细胞和CD8 + 3细胞的相对百分比增加，而T细胞、细胞毒性T细胞、活化T辅助细胞和大颗粒淋巴细胞（CD56 + 3 +）减少。当按性别和种族对CB样本进行分层时，也发现了显著差异。这些结果为CB淋巴细胞标志物提供了临床实验室正常参考值，证明了为显著不同的患者群体确定单独标准参考值的必要性，为未来病理性妊娠的研究和临床实验室应用提供了基础，并为早期免疫发育提供了见解。
