一种测量B族链球菌对小鼠腹腔渗出巨噬细胞黏附性的定量方法。

A quantitative method for measuring the adherence of group B streptococci to murine peritoneal exudate macrophages.

作者信息

Sloan A R, Pistole T G

机构信息

Department of Microbiology, University of New Hampshire, Durham 03824-3544.

出版信息

J Immunol Methods. 1992 Oct 2;154(2):217-23. doi: 10.1016/0022-1759(92)90195-y.

DOI:10.1016/0022-1759(92)90195-y

PMID:1401955

Abstract

We have developed a solid phase, direct binding, enzyme-linked immunosorbent assay (ELISA) to detect and quantify the adherence of group B streptococci to murine macrophages. The assay correlated well with direct microscopic quantification of adherence. As few as 3.8 x 10(4) bacteria/assay well or less than one bacterium per macrophage could be detected. This assay is both quantitative and selective, and is readily adaptable for multiple sample analysis. It provides a valuable alternative to visual detection of bacterial adherence.

摘要

我们开发了一种固相直接结合酶联免疫吸附测定法（ELISA），用于检测和定量B族链球菌对小鼠巨噬细胞的黏附。该测定法与黏附的直接显微镜定量结果相关性良好。每次测定孔中低至3.8×10⁴个细菌或每个巨噬细胞少于一个细菌都能被检测到。该测定法兼具定量性和选择性，易于适用于多个样本分析。它为细菌黏附的视觉检测提供了一种有价值的替代方法。