Peotta V A, Gomes F L, Arnholdt A C, Nagao P E
Departmento de Biologia Celular e Genética, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524-PHLC, 2. andar, Maracanã, Rio de Janeiro, RJ 20550-013, Brazil.
Curr Microbiol. 2001 Jul;43(1):64-8. doi: 10.1007/s002840010261.
In this report group B streptococci (GBS) strains 90356 and 80340 isolated from liquor and vagina, respectively, were placed into contact with human peripheral blood monocytes (PBM) and macrophages derived from monocytes (MDM) by differentiation in vitro. The increased expression of CD16 and CD68 by macrophages cultured for 7 days compared with adherent monocytes supported the distinct maturation status of these cells. The number of viable intracellular bacteria of the 90356 strain was observed after 2 h of incubation with PBM (P < 0.001) and 0.5 h with MDM (P < 0.001). MDM cells seemed to present a more efficient mechanism of bacterial destruction of GBS type III, isolated from a case of meningitis. Viable cells of strain 80340, isolated from the vagina, were not detected in significant numbers in PBM and MDM phagocytic cells. These findings add to our current understanding of the roles played by multiple receptor-ligand systems in the uptake and pathogenesis of group B streptococci infection. Survival strategies of GBS, which interfere with macrophage bactericidal functions, might exist.
在本报告中，分别从羊水和阴道分离出的B族链球菌（GBS）菌株90356和80340，通过体外分化与人类外周血单核细胞（PBM）以及由单核细胞分化而来的巨噬细胞（MDM）进行接触。与贴壁单核细胞相比，培养7天的巨噬细胞CD16和CD68表达增加，这支持了这些细胞不同的成熟状态。在与PBM孵育2小时（P < 0.001）和与MDM孵育0.5小时（P < 0.001）后，观察到90356菌株的存活细胞内细菌数量。MDM细胞似乎呈现出一种更有效的机制来破坏从一例脑膜炎病例中分离出的III型GBS。从阴道分离出的80340菌株的活细胞，在PBM和MDM吞噬细胞中未检测到大量存在。这些发现加深了我们目前对多种受体 - 配体系统在B族链球菌感染的摄取和发病机制中所起作用的理解。可能存在干扰巨噬细胞杀菌功能的GBS生存策略。
