Kline D L, Reddy K N
J Lab Clin Med. 1977 May;89(5):1153-8.
A procedure is presented for the caseinolytic assay of plasminogen proactivator and activator levels in blood. The validity of the method was established and normal plasma proactivator levels were determined in 10 subjects. No detectible activator activity was found in these subjects. Venous occulsion produced a small rise in activator activity. Hageman-deficient and Fitzgerald trait plasmas did not produce activator activity after exposure to kaolin. The generation of activator activity in the Fitzgerald plasma was partly restored by the addition of purified high molecular weight kininogen, but the Hageman-deficient plasma was not corrected. The caseinolytic assay avoids some of the drawbacks which are inherent in methods which depend on fibrin dissolution for the detection of enhanced fibrinolytic states, provides a reproducible baseline, and may be sufficiently sensitive to detect changes of clinical interest.
本文介绍了一种用于检测血液中纤溶酶原激活物和激活剂水平的酪蛋白溶解试验方法。该方法的有效性得到了验证，并测定了10名受试者的正常血浆激活物水平。在这些受试者中未发现可检测到的激活剂活性。静脉阻塞使激活剂活性略有升高。接触高岭土后，缺乏哈格曼因子和菲茨杰拉德特征的血浆未产生激活剂活性。添加纯化的高分子量激肽原可部分恢复菲茨杰拉德血浆中激活剂活性的产生，但缺乏哈格曼因子的血浆未得到纠正。酪蛋白溶解试验避免了一些依赖纤维蛋白溶解来检测纤维蛋白溶解状态增强的方法所固有的缺点，提供了一个可重复的基线，并且可能足够灵敏以检测具有临床意义的变化。
