Tange Y, Kanemitsu N, Okada T, Kobayashi Y, Tsuboi T
Department of Internal Medicine, School of Medicine, Ehime University.
Kansenshogaku Zasshi. 1992 Aug;66(8):1015-21. doi: 10.11150/kansenshogakuzasshi1970.66.1015.
Five strains of Rickettsia tsutsugamushi were isolated in Ehime Prefecture during December 1987 to January 1990. Of these, two strains, the Yamazaki and Noma-3, were isolated at Noma area of Imabari city and three strains, the Kakiwara-10, -11, -12, at Kakiwara area of Uwajima city. The Yamazaki strain was isolated from a patient of tsutsugamushi disease and the other strains from wild rodents (Apodemus speciosus). These strains showed virulence in euthymic mice. The calculated LD50 of the Yamazaki and Kakiwara-10 strains showed 10(-3.0) and 10(-1.8), respectively. The immunofluorescent antibody test using thirty monoclonal antibodies to six representative strains, the Gilliam, Karp, Kato, Irie, Hirano and Shimokoshi, revealed that two strains isolated at Noma area, the Yamazaki and Noma-3, were identified as the Karp type and three strains at Kakiwara area, the Kakiwara-10, -11, -12, were identified as the Kato type. It was clarified that the serotypic differences were present among the strains isolated in Ehime Prefecture. Moreover, these five strains isolated in Ehime Prefecture did not react with the serotype-specific monoclonal antibodies to the Irie, Hirano and Shimokoshi strains known as the representative strains of so-called new type of tsutsugamushi disease, showing antigenic differences.
1987年12月至1990年1月期间，在爱媛县分离出5株恙虫病东方体。其中，山崎株和野间-3株在今治市野间地区分离得到，垣原-10、-11、-12株在宇和岛市垣原地区分离得到。山崎株从恙虫病患者分离得到，其他株从野生啮齿动物（日本姬鼠）分离得到。这些菌株在正常小鼠中显示出毒力。计算得出山崎株和垣原-10株的半数致死量分别为10^(-3.0)和10^(-1.8)。使用针对6种代表性菌株（吉列姆、卡尔普、加藤、入江、平野和下越）的30种单克隆抗体进行免疫荧光抗体试验，结果显示在野间地区分离得到的2株（山崎株和野间-3株）被鉴定为卡尔普型，在垣原地区分离得到的3株（垣原-10、-11、-12株）被鉴定为加藤型。明确了爱媛县分离出的菌株之间存在血清型差异。此外，爱媛县分离出的这5株菌株与针对所谓新型恙虫病代表性菌株入江、平野和下越菌株的血清型特异性单克隆抗体不发生反应，显示出抗原性差异。
