Tamura A, Takahashi K, Tsuruhara T, Urakami H, Miyamura S, Sekikawa H, Kenmotsu M, Shibata M, Abe S, Nezu H
Microbiol Immunol. 1984;28(8):873-82. doi: 10.1111/j.1348-0421.1984.tb00743.x.
Rickettsia tsutsugamushi strains from three recent patients of Tsutsugamushi disease in Niigata Prefecture were isolated primarily in mice and then in L cell cultures. By this procedure, low virulent strains to mice, as well as high virulent ones, could be isolated and cultivated serially in L cell cultures, suggesting the usefulness of L cells for isolation of this species of rickettsia. Each newly isolated strain was identified as a member of R. tsutsugamushi from the results of cross immunological tests and morphological observation. On the other hand, it was recognized that one of these rickettsiae showed immunological properties distinguishable from the prototype strains of Kato, Karp, and Gilliam by the cross complement fixation test, and also had low virulence in mice.
从新潟县最近3例恙虫病患者身上分离出的恙虫病东方体菌株，首先在小鼠体内进行分离，然后在L细胞培养物中培养。通过这种方法，对小鼠低毒力菌株以及高毒力菌株都能进行分离，并能在L细胞培养物中连续传代培养，这表明L细胞在分离这种立克次氏体方面具有实用性。根据交叉免疫试验和形态学观察结果，每个新分离的菌株都被鉴定为恙虫病东方体的成员。另一方面，通过交叉补体结合试验发现，其中一种立克次氏体表现出与Kato、Karp和Gilliam原型菌株不同的免疫特性，并且在小鼠体内毒力较低。
