CLEMENTI F, ZOCCHE G P
J Cell Biol. 1963 Jun;17(3):587-96. doi: 10.1083/jcb.17.3.587.
Adrenomedullary cells, after fixation with OsO(4), are filled with well formed granules which are considered to represent their catechol amine content. The submicroscopic appearance of these cells was studied in reserpine-treated rats during the late phase of catechol amine depletion and during the period of its restoration. At 3 days after the beginning of reserpine treatment, the granules appeared to be emptied of their content and small vesicles containing scattered, dense deposits of, presumably, catechol amines began to be seen. At 9 days after the beginning of treatment, these deposits had already become granules and the cells had attained a completely normal appearance. The submicroscopic structure of the adrenomedullary cells of rats pretreated with iproniazid (before reserpine), in which a complete inhibition of monoamine oxidase activity had thus been obtained, was similar to that seen in non-treated animals. In numerous cases, however, some characteristic features were noted: the sacs which usually contained a dense granule of catechol amines appeared swollen and many fine granules could be seen around them; the latter were dispersed in a way suggesting that they may represent a partial breakdown of the large granules which, under the inhibitory action of iproniazid, do not release the catechol amines contained within them.
肾上腺髓质细胞经四氧化锇固定后，充满了形态良好的颗粒，这些颗粒被认为代表了它们的儿茶酚胺含量。在利血平处理的大鼠中，研究了儿茶酚胺耗竭后期及其恢复期间这些细胞的亚微观外观。在利血平治疗开始后3天，颗粒似乎已排空其内容物，开始出现含有散在的、可能是儿茶酚胺致密沉积物的小泡。在治疗开始后9天，这些沉积物已变成颗粒，细胞已恢复完全正常的外观。用异烟酰异丙肼预处理（在利血平之前）的大鼠肾上腺髓质细胞的亚微观结构，其中单胺氧化酶活性已完全受到抑制，与未处理动物的相似。然而，在许多情况下，注意到一些特征：通常含有儿茶酚胺致密颗粒的囊泡似乎肿胀，并且在它们周围可以看到许多细颗粒；后者的分散方式表明它们可能代表大颗粒的部分分解，在异烟酰异丙肼的抑制作用下，大颗粒不会释放其中所含的儿茶酚胺。
