Freyrie A, Curti T, Rodio M, Masetti L, Bignozzi L, Sanguinetti V, Joechler M, D'Addato M
Institute of Vascular Surgery, School of Veterinary, University of Bologna, Italy.
Int Angiol. 1992 Apr-Jun;11(2):113-6.
Infections caused by synthetic prostheses are relatively rare (1.5-6%) but serious complication in vascular surgery. There is no doubt that during and immediately after surgery bacterial contamination may occur. An in vitro study was carried out in the Vascutek laboratories, which revealed a high affinity between prostheses in Dacron gel and Rifampicin. This affinity, the result of an ionic bond, was demonstrated by the fact that after 5 days Rifampicin was still present on the prostheses. Encouraged by this result, an experimental study was carried out in sheep. Five sheep were operated on making a prosthetic graft in both of the common carotid arteries: on one side a Gelseal Dacron prosthesis was implanted after being soaked for 15 minutes in a solution containing 1 mg/ml Rifampicin. A Knitted Dacron prosthesis was implanted in the contralateral carotid artery, again after pretreatment with Rifampicin. Explants were made after 2, 24, 48, 72 and 96 hours, and the concentration of Rifampicin on the prostheses was assessed on the basis of the diameter of the inhibition area on Staphylococcus aureus cultures. The results showed that the Gelseal Dacron prostheses maintained Rifampicin concentrations with an antibacterial activity up to 72 hours; this property disappears with the Knitted Dacron prostheses after only 24 hours. These results confirm the high affinity of Gelseal Dacron and Rifampicin also in in vivo experimental models.
合成假体引起的感染相对少见（1.5 - 6%），但却是血管外科的严重并发症。毫无疑问，手术期间及术后即刻可能发生细菌污染。在Vascutek实验室进行了一项体外研究，该研究揭示了涤纶凝胶假体与利福平之间具有高度亲和力。这种亲和力是离子键作用的结果，5天后假体上仍有利福平这一事实证明了这一点。受此结果鼓舞，在绵羊身上开展了一项实验研究。对5只绵羊进行手术，在双侧颈总动脉制作假体移植物：一侧植入经1毫克/毫升利福平溶液浸泡15分钟后的Gelseal涤纶假体。对侧颈动脉植入针织涤纶假体，同样经过利福平预处理。分别在2、24、48、72和96小时后取出样本，根据金黄色葡萄球菌培养物上抑菌圈的直径评估假体上利福平的浓度。结果显示，Gelseal涤纶假体在72小时内维持具有抗菌活性的利福平浓度；而针织涤纶假体仅在24小时后就失去了这一特性。这些结果也证实了在体内实验模型中Gelseal涤纶与利福平之间具有高度亲和力。
