Black R S, Bouvier M M, Sheu K F, Darzynkiewicz Z, Blass J P
Altschul Laboratory for Dementia Research, Department of Neurology, Cornell University Medical College, Burke Medical Research Institute, White Plains, NY 10605.
J Neurol Sci. 1992 Aug;111(1):104-12. doi: 10.1016/0022-510x(92)90118-5.
Typical markers for neurons but not for astroglia have been identified in cells cultured from a sample of normal adult human temporal lobe, which was removed to gain access to a glioma. Cells were grown in medium containing growth factors, including fibroblast growth factor and nerve growth factor. The cells grew slowly (doubling time, 18 days) and have been carried as far as passage 8 over 10 months. Both immunoblotting and immunocytochemistry with redundant antibodies demonstrated the presence of neurofilaments (NF-H, NF-M, NF-L), but not glial fibrillary acidic protein (GFAP). Neuron-specific enolase (NSE) was also found. Morphologically, the cultures consisted of a pleimorphic population of cells with frequent long processes. Cells demonstrating neuronal rather than astroglial markers can be cultured from normal adult human brain.
在从一名正常成人大脑颞叶样本中培养的细胞中，已鉴定出典型的神经元标志物而非星形胶质细胞标志物。该样本是为了切除胶质瘤而获取的。细胞在含有生长因子（包括成纤维细胞生长因子和神经生长因子）的培养基中生长。细胞生长缓慢（倍增时间为18天），在10个月内已传至第8代。使用多种冗余抗体进行的免疫印迹和免疫细胞化学分析均显示存在神经丝（NF-H、NF-M、NF-L），但不存在胶质纤维酸性蛋白（GFAP）。还发现了神经元特异性烯醇化酶（NSE）。从形态学上看，培养物由具有频繁长突起的多形性细胞群体组成。可以从正常成人大脑中培养出显示神经元而非星形胶质细胞标志物的细胞。
