DeGiorgio L A, Sheu K F, Blass J P
Will Rogers Institute, Burke Medical Research Institute, Cornell University Medical College, White Plains, NY 10605.
J Neurol Sci. 1994 Jul;124(2):141-8. doi: 10.1016/0022-510x(94)90319-0.
Actively dividing cells cultured from human leptomeninges obtained at autopsy and from human pia obtained at neurosurgery expressed two antigens characteristic of a mature neuronal phenotype: the 160 kDa neurofilament protein (NF-M) and neuron-specific enolase (NSE). The cells lacked typical glial, epithelial and endothelial markers but did contain vimentin, a protein normally associated with but not limited to mesenchymally derived cell types. Immunocytochemical results using redundant antibodies were consistent over serial passage and were confirmed by Western blot analysis. Morphologically the cells were pleiomorphic with frequent long, NF-M + processes. The antigenic characteristics of the cultured leptomeningeal and pial cells were similar to those exhibited by human autopsy and biopsy brain-derived cultures described previously, suggesting that the latter may have originated from pial cells adherent to the autopsy and biopsy brain tissue specimens.
160 kDa神经丝蛋白（NF-M）和神经元特异性烯醇化酶（NSE）。这些细胞缺乏典型的神经胶质、上皮和内皮标志物，但确实含有波形蛋白，一种通常与间充质来源的细胞类型相关但不限于该类型的蛋白质。使用多种抗体的免疫细胞化学结果在连续传代中保持一致，并通过蛋白质印迹分析得到证实。形态学上，这些细胞多形性，常有长的、NF-M阳性的突起。培养的软脑膜和软膜细胞的抗原特征与先前描述的源自人类尸检和活检脑的培养物所表现出的特征相似，这表明后者可能起源于附着在尸检和活检脑组织标本上的软膜细胞。
