KAPLAN M T, APPLEMAN M D
Appl Microbiol. 1963 Jan;11(1):69-74. doi: 10.1128/am.11.1.69-74.1963.
Goldfish died within 24 hr after intraperitoneal injections of 0.2 ml of Seitz filtrates of hemolytic Staphylococcus aureus cultures grown on Dolman and Wilson medium under increased CO(2) pressure for 72 to 96 hr. Two lethal toxins differing in heat sensitivity, antigenicity, and degree of toxicity were demonstrated. Studies of the relationship between the lethal factors and the hemolysins in the filtrates suggested that alpha- and beta-lysin were responsible for the lethal effects. Filtrates of nonhemolytic staphylococcal cultures were innocuous. Goldfish were suitable animals for detecting toxicity in staphylococcal culture filtrates and for quantitative studies of the toxins. The results were highly reproducible.
在二氧化碳压力增加的条件下，将溶血性金黄色葡萄球菌培养物在多尔曼和威尔逊培养基上培养72至96小时后，取其Seitz滤液0.2毫升进行腹腔注射，金鱼在24小时内死亡。结果证明存在两种热敏感性、抗原性和毒性程度不同的致死毒素。对滤液中致死因子与溶血素之间关系的研究表明，α-溶血素和β-溶血素是致死效应的原因。非溶血性葡萄球菌培养物的滤液无毒。金鱼是检测葡萄球菌培养滤液毒性和毒素定量研究的合适动物。结果具有高度可重复性。
