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免疫荧光法检测红细胞上葡萄球菌α毒素时的特异性和非特异性染色

Specific and nonspecific staining in detecting staphylococcal alpha toxin on erythrocytes by immunofluorescence.

作者信息

Kaplan M T

出版信息

Infect Immun. 1972 May;5(5):831-2. doi: 10.1128/iai.5.5.831-832.1972.

DOI:10.1128/iai.5.5.831-832.1972
PMID:4118044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC422447/
Abstract

Specifically stained staphylococcal alpha toxin particles were detected on unstained and nonspecifically stained ghost membranes when rabbit erythrocytes were exposed to alpha toxin and stained with fluorescein isothiocyanate-labeled specific antitoxin. Fading of the nonspecific staining was observed and was attributed to the degradation of the membranes by alpha toxin.

摘要

当兔红细胞暴露于α毒素并用异硫氰酸荧光素标记的特异性抗毒素染色时,在未染色和非特异性染色的血影膜上检测到特异性染色的葡萄球菌α毒素颗粒。观察到非特异性染色褪色,这归因于α毒素对膜的降解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a7f/422447/afac04a86269/iai00269-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a7f/422447/fb9187021877/iai00269-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a7f/422447/afac04a86269/iai00269-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a7f/422447/fb9187021877/iai00269-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a7f/422447/afac04a86269/iai00269-0196-a.jpg

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本文引用的文献

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Science. 1964 Aug 14;145(3633):714-5. doi: 10.1126/science.145.3633.714.
2
FRACTIONATION OF STAPHYLOCOCCAL TOXINS BY GEL-FILTRATION.通过凝胶过滤法分离葡萄球菌毒素
Acta Pathol Microbiol Scand. 1963;59:543-52. doi: 10.1111/j.1699-0463.1963.tb01258.x.
3
Toxic effect of staphylococcal lysins for goldfish.葡萄球菌溶血素对金鱼的毒性作用。
Appl Microbiol. 1963 Jan;11(1):69-74. doi: 10.1128/am.11.1.69-74.1963.
4
Enhancement of immunofluorescent staining of erythrocytes by saponin hemolysis.皂苷溶血增强红细胞的免疫荧光染色。
Transfusion. 1965 Sep-Oct;5(5):434-9. doi: 10.1111/j.1537-2995.1965.tb02921.x.