免疫荧光法检测红细胞上葡萄球菌α毒素时的特异性和非特异性染色
Specific and nonspecific staining in detecting staphylococcal alpha toxin on erythrocytes by immunofluorescence.
作者信息
Kaplan M T
出版信息
Infect Immun. 1972 May;5(5):831-2. doi: 10.1128/iai.5.5.831-832.1972.
Abstract
Specifically stained staphylococcal alpha toxin particles were detected on unstained and nonspecifically stained ghost membranes when rabbit erythrocytes were exposed to alpha toxin and stained with fluorescein isothiocyanate-labeled specific antitoxin. Fading of the nonspecific staining was observed and was attributed to the degradation of the membranes by alpha toxin.
摘要
当兔红细胞暴露于α毒素并用异硫氰酸荧光素标记的特异性抗毒素染色时，在未染色和非特异性染色的血影膜上检测到特异性染色的葡萄球菌α毒素颗粒。观察到非特异性染色褪色，这归因于α毒素对膜的降解。
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