Logan A, Frautschy S A, Gonzalez A M, Baird A
Department of Clinical Chemistry, University of Birmingham, United Kingdom.
J Neurosci. 1992 Oct;12(10):3828-37. doi: 10.1523/JNEUROSCI.12-10-03828.1992.
Traumatic injury to the CNS initiates transient and unsuccessful regeneration of damaged neural pathways, accompanied by reactive gliosis, angiogenesis, and deposition of a dense fibrous glial/meningeal scar at the wound site. Basic fibroblast growth factor (basic FGF) is a CNS protein with potent effects on neurons, glia, fibroblasts, and vascular endothelial cells. Hybridization and immunocytochemical methods were used to examine temporal and spatial changes in distribution and levels of basic FGF protein and mRNA and also of its receptor mRNA (flg), following a defined wound to the cerebral cortex of adult rat brains. In the injured brain, a rapid, transient increase in basic FGF mRNA and protein is readily detectable within 7 d of surgery and thereafter declines in the tissues bordering the lesion. The increased expression is localized to multiple cell types including macrophages, neurons, astrocytes, and vascular endothelial cells. The changes in immunoreactive basic FGF parallel changes in the bioactivity of extracted heparin-binding proteins, which include basic FGF. Focal increases in flg mRNA appear 7 d after injury and subside by 14 d. The changes in local basic FGF synthesis, concentration, localization, and bioactivity suggest that this growth factor may contribute to the cascade of cellular events that occur in CNS wound repair.
中枢神经系统（CNS）的创伤性损伤会引发受损神经通路的短暂且不成功的再生，同时伴有反应性胶质增生、血管生成以及在伤口部位形成致密的纤维性胶质/脑膜瘢痕。碱性成纤维细胞生长因子（碱性FGF）是一种对神经元、神经胶质细胞、成纤维细胞和血管内皮细胞有强大作用的中枢神经系统蛋白质。采用杂交和免疫细胞化学方法，研究成年大鼠大脑皮质特定伤口后碱性FGF蛋白和mRNA及其受体mRNA（flg）的分布和水平的时空变化。在受伤的大脑中，手术7天内即可轻易检测到碱性FGF mRNA和蛋白迅速短暂增加，之后在损伤周边组织中下降。表达增加定位于多种细胞类型，包括巨噬细胞、神经元、星形胶质细胞和血管内皮细胞。免疫反应性碱性FGF的变化与包括碱性FGF在内的提取的肝素结合蛋白的生物活性变化平行。flg mRNA在损伤7天后出现局部增加，并在14天时消退。局部碱性FGF合成、浓度、定位和生物活性的变化表明，这种生长因子可能有助于中枢神经系统伤口修复中发生的一系列细胞事件。
