TRAXLER R W, LANKFORD C E, SHIVE W
Appl Microbiol. 1962;10(2):99-101. doi: 10.1128/am.10.2.99-101.1962.
A procedure is described whereby the growth stimulatory material from an autoclaved solution of glucose and phosphate can routinely be purified 689-fold. Chemical characterization of this purified material indicates the presence of a hydroxyl group and possibly a carboxyl group on the active factor(s) which are associated with activity. Evidence is presented that there are two active factors or two forms of the same factor present in the concentrate.
本文描述了一种方法，通过该方法可将葡萄糖和磷酸盐高压灭菌溶液中的生长刺激物质常规纯化689倍。对该纯化物质的化学表征表明，活性因子上存在羟基，可能还存在羧基，这些与活性相关。有证据表明，浓缩物中存在两种活性因子或同一因子的两种形式。
