BLIZZARD J L, PETERSON G E
J Bacteriol. 1963 May;85(5):1136-40. doi: 10.1128/jb.85.5.1136-1140.1963.
Blizzard, John L. (University of Houston, Houston, Texas) and G. E. Peterson. Selective inhibition of proline-induced pigmentation in washed cells of Serratia marcescens. J. Bacteriol. 85:1136-1140. 1963.-Streptomycin, chloramphenicol, and tetracyclines inhibited the synthesis of prodigiosin by Serratia marcescens strain D1. This occurred at concentrations of the antibiotic too low to inhibit the growth of the organism in either agar media or broth cultures. Nonpigmented cells were produced in broth by either adding streptomycin or incubating at 37 C. After being washed and resuspended in aqueous saline containing either casein hydrolysate, l-proline, or a glycine-succinate mixture and incubated at 27 C for 24 hr, these cells formed pigment. The appearance of pigment was preceded by a lag period of 10 hr. Prodigiosin production by these washed suspensions of cells was completely inhibited by either streptomycin or glucose, or by incubation at 37 C instead of 27 C. Even though pigmentation by washed-cell suspensions was induced by proline, the utilization of proline was not affected by streptomycin or glucose, or by incubation at 37 C. To block pigmentation completely, streptomycin had to be added to proline-supplemented cells before they were 10 hr old. Addition of the antibiotic after the end of the induction period caused either partial or no inhibition of pigment production. Streptomycin caused an increase in the endogenous respiration of S. marcescens but failed to affect the constitutive enzymes that oxidize glucose. The possible relationships of these phenomena are discussed. Weil (1952) reported that low concentrations of chloramphenicol and certain tetracyclines inhibit the synthesis of prodigiosin while permitting growth by Serratia marcescens. He noted the potential value to "mode-of-action" studies of an organism having certain functions selectively inhibited by antibiotics. We confirmed Weil's (1952) observations and found that streptomycin at low concentration would also inhibit the synthesis of prodigiosin without impeding growth. Further studies of the selective inhibition of prodigiosin synthesis by streptomycin were performed using nonproliferating suspensions of washed cells (Gott and Williams, 1961). Either a glycine-succinate mixture or l-proline could cause nonproliferating cells to form pigment. A period of induction preceded the formation of pigment. Either streptomycin or glucose, or an incubation temperature of 37 C, inhibited the proline-induced pigmentation by washed cells. Further investigations provided insights to these findings.
布莱兹德，约翰·L.（得克萨斯州休斯敦市休斯敦大学）和G.E.彼得森。粘质沙雷氏菌洗涤细胞中脯氨酸诱导色素沉着的选择性抑制。《细菌学杂志》85:1136 - 1140。1963年。-链霉素、氯霉素和四环素抑制了粘质沙雷氏菌D1菌株灵菌红素的合成。这种情况发生在抗生素浓度过低以至于无法在琼脂培养基或肉汤培养物中抑制该生物体生长的情况下。通过添加链霉素或在37℃培养可在肉汤中产生无色素细胞。将这些细胞洗涤后重悬于含有酪蛋白水解物、L - 脯氨酸或甘氨酸 - 琥珀酸盐混合物的盐水溶液中，并在27℃培养24小时后，这些细胞形成色素。色素出现之前有10小时的延迟期。这些洗涤后的细胞悬液产生灵菌红素的过程被链霉素或葡萄糖完全抑制，或者通过在37℃而非27℃培养来抑制。尽管洗涤细胞悬液的色素沉着是由脯氨酸诱导的，但脯氨酸的利用不受链霉素或葡萄糖的影响也不受在37℃培养的影响。为了完全阻断色素沉着，必须在脯氨酸补充的细胞10小时龄之前添加链霉素。在诱导期结束后添加抗生素会导致色素产生部分抑制或无抑制。链霉素导致粘质沙雷氏菌的内源性呼吸增加，但未能影响氧化葡萄糖的组成酶。讨论了这些现象可能的关系。韦尔（1952年）报道低浓度的氯霉素和某些四环素抑制灵菌红素的合成，同时允许粘质沙雷氏菌生长。他指出了对于“作用模式”研究而言，一种具有某些功能被抗生素选择性抑制的生物体的潜在价值。我们证实了韦尔（1952年）的观察结果，并发现低浓度的链霉素也会抑制灵菌红素的合成而不阻碍生长。使用洗涤细胞的非增殖悬液（戈特和威廉姆斯，1961年）对链霉素对灵菌红素合成的选择性抑制进行了进一步研究。甘氨酸 - 琥珀酸盐混合物或L - 脯氨酸都可使非增殖细胞形成色素。色素形成之前有一段诱导期。链霉素或葡萄糖，或者37℃的培养温度，抑制了洗涤细胞中脯氨酸诱导的色素沉着。进一步的研究为这些发现提供了深入见解。
