Williams R P
Appl Microbiol. 1973 Mar;25(3):396-402. doi: 10.1128/am.25.3.396-402.1973.
Prodigiosenes (prodigiosin and prodigiosin-like pigments) are known to be synthesized by only one genus of Eubacteriales and by two genera of Actinomycetales. Biosynthesis by Serratia marcescens occurs over a relatively narrow range of temperatures, although the bacteria grow over a broad range. When cultures of S. marcescens were incubated at 27 C in 1.0% casein hydrolysate, viable count and protein attained maximal values within 24 to 48 h, whereas prodigiosin did not reach a maximum until 96 h. The greatest amount of pigment was synthesized when cultures were in the senescent phase of growth. Suspensions of nonproliferating bacteria incubated at 27 C in only L-alanine also synthesized prodigiosin, although at a slower rate than growing cultures. Kinetics of growth for the wild-type, red S. marcescens and a white mutant were identical when incubated at 27 C, but the wild type produced abundant pigment. These results plus other data obtained from the literature suggest that prodigiosin is a secondary metabolite. The importance of this proposal to understanding the function of prodigiosin in S. marcescens is discussed.
灵菌红素（灵菌红素及类灵菌红素色素）已知仅由真细菌目一个属及放线菌目两个属合成。尽管粘质沙雷氏菌能在较宽温度范围内生长，但其灵菌红素的生物合成发生在相对较窄的温度范围内。当粘质沙雷氏菌培养物在27℃下于1.0%酪蛋白水解物中培养时，活菌数和蛋白质在24至48小时内达到最大值，而灵菌红素直到96小时才达到最大值。当培养物处于生长衰老期时，合成的色素量最大。在仅含L - 丙氨酸的培养基中于27℃培养的非增殖细菌悬液也能合成灵菌红素，不过合成速率比生长中的培养物慢。野生型红色粘质沙雷氏菌和白色突变体在27℃培养时生长动力学相同，但野生型产生大量色素。这些结果以及从文献中获得的其他数据表明灵菌红素是一种次生代谢产物。本文讨论了这一观点对于理解灵菌红素在粘质沙雷氏菌中的功能的重要性。
