FALCONE G, NICKERSON W J
J Bacteriol. 1963 Apr;85(4):754-62. doi: 10.1128/jb.85.4.754-762.1963.
Falcone, Giuseppe (Rutgers, The State University, New Brunswick, N.J.), and Walter J. Nickerson. Reduction of selenite by intact yeast cells and cell-free preparations. J. Bacteriol. 85:754-762. 1963.-Nonproliferating cell suspensions of Candida albicans rapidly reduced selenite to red, metallic selenium in the absence of added substrate. Cell suspensions reduced selenite optimally at pH 4.2. No added metabolite was found to be stimulatory; reduction was inhibited by methionine and formate as well as by fluoride, dinitrophenol, and certain sulfhydryl poisons. Cell-free preparations capable of reducing selenite were obtained from C. albicans and from baker's yeast disintegrated in a Hughes press. The enzymatic system had optimal activity at pH 7 with 10(-2)m selenite. Activity of the system was lost on dialysis but was restored upon the addition of dialyzable substances or of boiled, undialyzed extract.
法尔科内，朱塞佩（罗格斯大学，新泽西州新不伦瑞克）和沃尔特·J·尼克森。完整酵母细胞和无细胞制剂对亚硒酸盐的还原作用。《细菌学杂志》85:754 - 762。1963年。 - 在没有添加底物的情况下，白色念珠菌的非增殖细胞悬液能迅速将亚硒酸盐还原为红色金属硒。细胞悬液在pH 4.2时对亚硒酸盐的还原效果最佳。未发现添加的代谢物具有刺激作用；蛋氨酸、甲酸以及氟化物、二硝基苯酚和某些巯基毒物会抑制还原作用。从白色念珠菌和在休斯压榨机中破碎的面包酵母中获得了能够还原亚硒酸盐的无细胞制剂。该酶系统在pH 7、亚硒酸盐浓度为10⁻²m时具有最佳活性。该系统的活性在透析后丧失，但添加可透析物质或煮沸的未透析提取物后可恢复。
