Fusegawa H, Miyachi H, Tsutsumi Y, Tokuda Y, Ando Y
Department of Clinical Pathology, Tokai University School of Medicine, Isehara.
Rinsho Byori. 1992 Aug;40(8):891-5.
In patients with cervical lymph node swelling, prompt differential diagnosis of tuberculosis leads to an appropriate treatment. A method based on DNA amplification and hybridization for the rapid detection of Mycobacterium tuberculosis was used for demonstrating its specific DNA in cervical lymph nodes biopsied from seven patients. The DNA specific for Mycobacterium tuberculosis was detected in 5 patients. Four of them were histologically diagnosed as tuberculous lymphadenitis. In another case with necrotizing lesions but without granulomatous reactions, culture method subsequently revealed a positive result. In other 2 cases, the diagnosis of tuberculosis was promptly excluded by both this method and conventional methods (histologic diagnosis/direct microscopy). The rapid detection of Mycobacterium tuberculosis by amplification of mycobacterial DNA is helpful particularly in differential diagnosis of cervical lymphadenopathy.
对于颈部淋巴结肿大的患者，及时对结核病进行鉴别诊断可实现恰当治疗。一种基于DNA扩增和杂交的快速检测结核分枝杆菌的方法，被用于检测7例患者颈部淋巴结活检组织中的结核分枝杆菌特异性DNA。5例患者检测到结核分枝杆菌特异性DNA。其中4例经组织学诊断为结核性淋巴结炎。在另一例有坏死性病变但无肉芽肿反应的病例中，培养方法随后显示为阳性结果。在另外2例病例中，该方法和传统方法（组织学诊断/直接显微镜检查）均迅速排除了结核病诊断。通过扩增分枝杆菌DNA快速检测结核分枝杆菌，尤其有助于颈部淋巴结病的鉴别诊断。
