Fusegawa H, Miyachi H, Ohshima T, Arimori S, Ando Y
Department of Clinical Pathology, Tokai University School of Medicine.
Rinsho Ketsueki. 1992 Apr;33(4):418-22.
In patients with malignant blood disease, infection is the most serious complication. The prompt differential diagnosis of tuberculosis leads to an appropriate treatment. A method based on DNA amplification and hybridization for the rapid detection of Mycobacterium tuberculosis was used to test 10 clinical specimens (sputum, gastric aspirate and pleural effusion) from blood disease patients in whom tuberculosis was suspected. Mycobacterium tuberculosis DNA was detected in 3 specimens, including one which was negative on standard microbiological criteria (microscopy and/or culture). The other 7 specimens with fever or abnormal shadow on a chest X-ray were negative by both our method and the Standard microbiological criteria. Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in cases of blood disease in clinically useful.
在恶性血液病患者中，感染是最严重的并发症。对结核病进行及时的鉴别诊断可实现恰当治疗。采用一种基于DNA扩增和杂交的快速检测结核分枝杆菌的方法，对10例疑似患有结核病的血液病患者的临床标本（痰液、胃吸出物和胸腔积液）进行检测。在3份标本中检测到结核分枝杆菌DNA，其中1份根据标准微生物学标准（显微镜检查和/或培养）为阴性。另外7份有发热或胸部X光片异常阴影的标本，通过我们的方法和标准微生物学标准检测均为阴性。在血液病病例中，通过扩增分枝杆菌DNA对结核病进行快速诊断具有临床实用性。
