Seo Y, Takahama K
Department of Legal Medicine, Miyazaki Medical College, Japan.
Nihon Hoigaku Zasshi. 1992 Jun;46(3):169-76.
We attempted to develop a method for the determination of liver injuries, using a liver-specific antigen as a marker. The liver-specific antigen (LSA) was purified from the human liver and the antibody to the human LSA only reacted with the liver extract using the immuno-dot-blotting technique. Depending on the immunohistochemical study, the LSA was found to be located within the cytoplasm of hepatocytes. A sensitive and specific sandwich enzyme immunoassay was then developed for the measurement of LSA. The detection limit of human LSA was 1 fmol/tube (52 pg/tube) and this assay was not affected by hemolysis. The LSA levels in serum and blood from healthy subjects were distributed within a range below the detection limit. The LSA levels in the blood from cadavers whose livers had been damaged, were markedly elevated in comparison with the normal levels found in other cadavers (10-140 fold). No cross-reaction was observed with the liver extracts from several species (mouse, rat, guinea pig and rabbit). These results suggest that the measurement of LSA levels in blood will become a useful marker for the detection of liver injury.
我们试图开发一种以肝脏特异性抗原作为标志物来测定肝损伤的方法。从人肝脏中纯化出肝脏特异性抗原（LSA），使用免疫斑点印迹技术，抗人LSA抗体仅与肝脏提取物发生反应。根据免疫组织化学研究，发现LSA位于肝细胞的细胞质内。随后开发了一种灵敏且特异的夹心酶免疫测定法来检测LSA。人LSA的检测限为1 fmol/管（52 pg/管），该测定法不受溶血影响。健康受试者血清和血液中的LSA水平分布在检测限以下的范围内。肝脏受损尸体血液中的LSA水平与其他尸体中的正常水平相比显著升高（10 - 140倍）。未观察到与几种物种（小鼠、大鼠、豚鼠和兔子）的肝脏提取物发生交叉反应。这些结果表明，测定血液中的LSA水平将成为检测肝损伤的有用标志物。
