Mihas A A, Saccomani G, Spenney J G
Division of Digestive Diseases, University of Mississippi School of Medicine, Jackson, USA.
J Med. 1994;25(5):285-303.
The immunological and biological properties of a liver-specific antigen (LSA) from rat liver are described. LSA gave interspecies cross-reactions with liver extracts from several mammalian species, but no reaction of complete identify was observed. Moreover, no cross-reaction was found with chicken or frog livers, thus indicating the rather late appearance of LSA in the process of evolution. Experiments with fetal and neonatal liver extracts have indicated that LSA appears late in fetal development and is always present at birth. The biological properties of LSA were explored by several independent approaches. LSAg-Ab immunoprecipitates were stained positively with carbon naphthoxycholine iodide, an indirect evidence of choline esterase activity. LSA was also found to bind bile acids, thus suggesting organic anion properties. Finally, LSA was detected in the circulation of rats with acute carbon tetrachloride- and galactosamine-induced hepatocellular injury. This LSA is immunologically distinct from hepatitis B antigen, alpha-fetoprotein, carcino-embryonic antigen and, from each of several serum and liver proteins tested.
本文描述了大鼠肝脏中一种肝脏特异性抗原（LSA）的免疫学和生物学特性。LSA与几种哺乳动物肝脏提取物发生种间交叉反应，但未观察到完全相同的反应。此外，未发现与鸡或青蛙肝脏有交叉反应，这表明LSA在进化过程中出现较晚。对胎儿和新生儿肝脏提取物的实验表明，LSA在胎儿发育后期出现，且出生时始终存在。通过几种独立的方法探究了LSA的生物学特性。LSAg-Ab免疫沉淀物用碘化碳萘氧胆碱染色呈阳性，这是胆碱酯酶活性的间接证据。还发现LSA能结合胆汁酸，因此提示其具有有机阴离子特性。最后，在急性四氯化碳和半乳糖胺诱导的肝细胞损伤大鼠的循环中检测到了LSA。这种LSA在免疫学上与乙肝抗原、甲胎蛋白、癌胚抗原以及所检测的几种血清和肝脏蛋白均不同。
