单核细胞增生李斯特菌溶血素的产生与性质
PRODUCTION AND NATURE OF LISTERIA MONOCYTOGENES HEMOLYSINS.
作者信息
NJOKU-OBI A N, JENKINS E M, NJOKU-OBI J C, ADAMS J, COVINGTON V
出版信息
J Bacteriol. 1963 Jul;86(1):1-8. doi: 10.1128/jb.86.1.1-8.1963.
Njoku-Obi, Augustine N. (School of Veterinary Medicine, Tuskegee Institute, Ala.), Edward M. Jenkins, Jessie C. Njoku-Obi, Joanne Adams, and Verdell Covington. Production and nature of Listeria monocytogenes hemolysins. J. Bacteriol. 86:1-8. 1963.-Hemolysin produced by various strains of Listeria monocytogenes varied in quality and quantity, depending on medium, incubation temperature and time, and biological variations in the organisms. The hemolysin was inactivated by filtration (through Seitz, Selas, or sintered-glass filters), heat, oxygen, and formalin. Sodium thiosulfate reactivated hemolysin inactivated by filtration and oxygen. The hemolysin was protein in nature, migrating electrophoretically as a gamma-globulin, and highly antigenic in the rabbit. Although no toxicity was observed in intact mice injected with hemolysin, a possible leukocytolysis was noted with isolated mice peritoneal exudate cells. Due to the high antihemolytic activity of normal sera from various species, the possible use of an antilisteriolysin test in serological diagnosis is questioned.
恩乔库 - 奥比，奥古斯丁·N.（阿拉巴马州塔斯基吉学院兽医学院），爱德华·M. 詹金斯，杰西·C. 恩乔库 - 奥比，乔安妮·亚当斯和韦德尔·科温顿。单核细胞增生李斯特菌溶血素的产生及性质。《细菌学杂志》86:1 - 8。1963年。 - 不同菌株的单核细胞增生李斯特菌产生的溶血素在质量和数量上有所不同，这取决于培养基、培养温度和时间以及生物体的生物学变异。溶血素可通过过滤（通过赛茨、塞拉斯或烧结玻璃过滤器）、加热、氧气和福尔马林使其失活。硫代硫酸钠可使因过滤和氧气失活的溶血素重新激活。溶血素本质上是蛋白质，在电泳中作为γ - 球蛋白迁移，并且在兔体内具有高度抗原性。尽管给完整小鼠注射溶血素未观察到毒性，但在分离的小鼠腹腔渗出细胞中注意到可能存在白细胞溶解现象。由于来自不同物种的正常血清具有高抗溶血活性，因此对在血清学诊断中使用抗李斯特菌溶血素试验的可能性提出了质疑。
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