Snyder I S, Koch N A
J Bacteriol. 1966 Feb;91(2):763-7. doi: 10.1128/jb.91.2.763-767.1966.
Snyder, Irvin S. (University of Iowa, Iowa City), and Nancy A. Koch. Production and characteristics of hemolysins of Escherichia coli. J. Bacteriol. 91:763-767. 1996.-Filterable and nonfilterable hemolysins were produced by Escherichia coli in beef-heart infusion, casein hydrolysate, and chemically defined media. Differences were found among the three media in the time of appearance and in the relationship between production of these two hemolysins. The nonfilterable hemolysin produced in the three media, as well as the filterable hemolysin produced in the beef-heart infusion medium, were destroyed in 1 hr at 56 C. The filterable hemolysin produced in the casein hydrolysate and chemically defined media was unaffected by exposure to 56 C for 1 hr. Formalin inactivated the hemolysins produced in all three media. The optimal pH for activity of the nonfilterable hemolysin varied with time of production, whereas the optimal pH for the filterable hemolysin was constant. Certain carbohydrates were required for the production of filterable hemolysin by E. coli grown in casein hydrolysate and chemically defined media.
斯奈德，欧文·S.（衣阿华大学，衣阿华市）和南希·A.科赫。大肠杆菌溶血素的产生及特性。《细菌学杂志》91:763 - 767。1996年。——大肠杆菌在牛心浸液、酪蛋白水解物和化学限定培养基中产生可过滤和不可过滤的溶血素。在这三种培养基中，这两种溶血素出现的时间以及产生量之间的关系存在差异。在三种培养基中产生的不可过滤溶血素，以及在牛心浸液培养基中产生的可过滤溶血素，在56℃下1小时内被破坏。在酪蛋白水解物和化学限定培养基中产生的可过滤溶血素在56℃下暴露1小时不受影响。福尔马林使在所有三种培养基中产生的溶血素失活。不可过滤溶血素活性的最适pH随产生时间而变化，而可过滤溶血素的最适pH是恒定的。在酪蛋白水解物和化学限定培养基中生长的大肠杆菌产生可过滤溶血素需要某些碳水化合物。
