MAGIE A R, WILSON E E, KOSUGE T
Science. 1963 Sep 27;141(3587):1281-2. doi: 10.1126/science.141.3587.1281.
When DL-tryptophan-2-C(14) was incubated with washed cells or cell-free preparations of Pseudomonas savastanoi, two radioactive metabolites were formed. One was identified as indoleacetamide and the second, indoleacetic acid. The amount of indoleacetamide in the reaction mixture increased rapidly during the early stages of incubation; it reached a peak after 15 minutes and declined steadily there-after. Indoleacetic acid, on the other hand, accumulated slowly throughout the incubation period. Cell-free preparations preferentially utilize the L-isomer of tryptophan for the synthesis of indoleacetamide and indoleacetic acid. The results of these experiments suggest, therefore, that P. savastanoi synthesizes indoleacetic acid by the following reactions: L-tryptophan--> indoleacetamide-->indoleacetic acid.
当DL-色氨酸-2-C(14)与洗涤过的细胞或野油菜黄单胞菌的无细胞制剂一起温育时，形成了两种放射性代谢物。一种被鉴定为吲哚乙酰胺，另一种是吲哚乙酸。反应混合物中吲哚乙酰胺的量在温育早期迅速增加；15分钟后达到峰值，此后稳步下降。另一方面，吲哚乙酸在整个温育期间缓慢积累。无细胞制剂优先利用色氨酸的L-异构体来合成吲哚乙酰胺和吲哚乙酸。因此，这些实验结果表明，野油菜黄单胞菌通过以下反应合成吲哚乙酸：L-色氨酸→吲哚乙酰胺→吲哚乙酸。
