Department of Plant Pathology, University of California, Davis, CA 95616.
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6522-6. doi: 10.1073/pnas.82.19.6522.
We report the nucleotide sequences of iaaM and iaaH, the genetic determinants for, respectively, tryptophan 2-monooxygenase and indoleacetamide hydrolase, the enzymes that catalyze the conversion of L-tryptophan to indoleacetic acid in the tumor-forming bacterium Pseudomonas syringae pv. savastanoi. The sequence analysis indicates that the iaaM locus contains an open reading frame encoding 557 amino acids that would comprise a protein with a molecular weight of 61,783; the iaaH locus contains an open reading frame of 455 amino acids that would comprise a protein with a molecular weight of 48,515. Significant amino acid sequence homology was found between the predicted sequence of the tryptophan monooxygenase of P. savastanoi and the deduced product of the T-DNA tms-1 gene of the octopine-type plasmid pTiA6NC from Agrobacterium tumefaciens. Strong homology was found in the 25 amino acid sequence in the putative FAD-binding region of tryptophan monooxygenase. Homology was also found in the amino acid sequences representing the central regions of the putative products of iaaH and tms-2 T-DNA. The results suggest a strong similarity in the pathways for indoleacetic acid synthesis encoded by genes in P. savastanoi and in A. tumefaciens T-DNA.
我们报道了 iaaM 和 iaaH 的核苷酸序列，分别为色氨酸 2-单加氧酶和吲哚乙酰胺水解酶的遗传决定簇，这些酶催化肿瘤形成菌丁香假单胞菌 pv. savastanoi 中 L-色氨酸向吲哚乙酸的转化。序列分析表明，iaaM 基因座包含一个开放阅读框，编码 557 个氨基酸，将组成一个分子量为 61783 的蛋白质；iaaH 基因座包含一个 455 个氨基酸的开放阅读框，将组成一个分子量为 48515 的蛋白质。在 P. savastanoi 的色氨酸单加氧酶的预测序列与农杆菌 octopine 型质粒 pTiA6NC 的 T-DNA tms-1 基因的推导产物之间发现了显著的氨基酸序列同源性。在色氨酸单加氧酶中假定的 FAD 结合区域的 25 个氨基酸序列中发现了强同源性。iaaH 和 tms-2 T-DNA 的假定产物的中心区域的氨基酸序列也存在同源性。结果表明，P. savastanoi 和农杆菌 T-DNA 中编码吲哚乙酸合成途径的基因具有很强的相似性。
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