Berger A, Gershwin M E, German J B
Department of Food Science and Technology, University of California, Davis 95616.
Lipids. 1992 Aug;27(8):605-12. doi: 10.1007/BF02536118.
Cardiolipin (CL) is a unique mitochondrial phospholipid, containing up to 85 wt% 18:2n-6 in mammals. The influence of maternal dietary fatty acids on the acyl composition of offspring CL has not been examined previously. Adult female mice were thus fed diets rich in 18:1n-9 (olive oil), 18:2n-6 (safflower oil), 18:3n-3 (linseed oil) or 20:5n-3 and 22:6n-3 (fish oil/safflower, 9:1, w/w), for a five month period, encompassing two breeding cycles. Offspring from the second breeding cycle were then fed these diets. The acyl composition of CL, phosphatidylcholine and phosphatidylethanolamine from liver and heart was evaluated from mice killed 3, 18 and 42 days after parturition. The primary nutrient sources at these three time points were transplacental nutrients, breast milk and the diet, respectively. Maternal diet was found to influence the acyl composition of CL via both placental transfer of fatty acids and breast milk. Fish oil feeding resulted in replacement of a substantial portion of 18:2n-6 with 22:6n-3; after 42 days, the area% of 18:2n-6 in heart CL was reduced from 62% in safflower oil fed mice to 12%. In comparison to fish oil feeding, linseed oil feeding resulted in a much lower accumulation of 22:6n-3. Olive oil feeding resulted in substantial replacement of 18:2n-6 with 18:1n-9 (18:2n-6 was reduced from 62% to 31%). Physiologically, these findings are relevant because changes in CL acyl composition may influence the activity of associated inner mitochondrial membrane enzymes.
心磷脂（CL）是一种独特的线粒体磷脂，在哺乳动物中含有高达85%（重量）的18:2n-6。母体饮食脂肪酸对后代心磷脂酰基组成的影响此前尚未得到研究。因此，成年雌性小鼠被喂食富含18:1n-9（橄榄油）、18:2n-6（红花油）、18:3n-3（亚麻籽油）或20:5n-3和22:6n-3（鱼油/红花油，9:1，重量比）的饮食，为期五个月，涵盖两个繁殖周期。然后，将第二个繁殖周期的后代喂食这些饮食。从分娩后3天、18天和42天处死的小鼠中评估肝脏和心脏中心磷脂、磷脂酰胆碱和磷脂酰乙醇胺的酰基组成。这三个时间点的主要营养来源分别是经胎盘的营养物质、母乳和饮食。发现母体饮食通过脂肪酸的胎盘转运和母乳影响心磷脂的酰基组成。喂食鱼油导致大量的18:2n-6被22:6n-3取代；42天后，心脏心磷脂中18:2n-6的面积百分比从喂食红花油小鼠的62%降至12%。与喂食鱼油相比，喂食亚麻籽油导致22:6n-3的积累要低得多。喂食橄榄油导致大量的18:2n-6被18:1n-9取代（18:2n-6从62%降至31%）。从生理角度来看，这些发现具有相关性，因为心磷脂酰基组成的变化可能会影响相关线粒体内膜酶的活性。
