Szaflarska-Stojko E, Olczyk D, Domagała B
Zakładu Doświadczalnej Patologii Pracy Instytutu Medycyny Pracy, Sosnowcu.
Med Pr. 1992;43(3):209-16.
In order to determine biological aggressiveness of settled dusts (mechanical, crumbled) and dusts collected using the gravimetric method, experimental studies were carried out, including: 1) evaluation of the physicochemical parameters (size of dust particles, content of silica, metals and other chemical compounds), 2) evaluation of the haemolytic activity, 3) experimental evaluation of fibrogenic potentials by means of: a) intraperitoneal test--to identity morphological type of reactive changes in the peritoneum and b) intratracheal test--to determine the level of hydroxyproline (collagen) in lungs and the morphological type of reactive changes. Albino rats were used for the experiment. The animals were divided into ten groups which received a single intratracheal injection of 50 mg of mining dust in 0.9% NaCl suspension. Comparative evaluation of biological aggressiveness of mining dusts was conducted basing on the findings of collagen levels in lungs. After the end of the experimental period (3 and 6 months, respectively) histopathological a examination of the lungs and mediastinal lymph nodes was made and the collagen levels in the pulmonary tissue (following Stegemann) were determined. As evidenced by the results of the pathomorphological examination and a statistical analysis: 1) after intratracheal injection the mining dusts induced changes within the respiratory system e.g. inflammatory process and emphysema. The exposure also brought about double increase of collagen level as compared to the control group, 2) histopathological study of the lungs and lymph nodes did not reveal progressive development of fibrogenic changes, 3) cytotoxic test showed differences in the haemolytic activity of settled dust and dusts collected by the gravimetric method.
为了确定沉降粉尘(机械粉碎的)和采用重量法收集的粉尘的生物侵袭性,开展了实验研究,包括:1)评估物理化学参数(粉尘颗粒大小、二氧化硅、金属及其他化合物的含量),2)评估溶血活性,3)通过以下方式对致纤维化潜能进行实验评估:a)腹腔内试验——以确定腹膜反应性变化的形态学类型,b)气管内试验——以测定肺中羟脯氨酸(胶原蛋白)水平及反应性变化的形态学类型。实验使用白化大鼠。将动物分为十组,每组接受一次气管内注射0.9%氯化钠悬浮液中50毫克的采矿粉尘。基于肺中胶原蛋白水平的研究结果对采矿粉尘的生物侵袭性进行了比较评估。在实验期结束后(分别为3个月和6个月),对肺和纵隔淋巴结进行了组织病理学检查,并测定了肺组织中的胶原蛋白水平(按照施特格曼法)。病理形态学检查结果和统计分析表明:1)气管内注射采矿粉尘后,呼吸系统出现了变化,如炎症过程和肺气肿。与对照组相比,暴露还使胶原蛋白水平增加了一倍,2)肺和淋巴结的组织病理学研究未发现致纤维化变化的进展,3)细胞毒性试验显示沉降粉尘和重量法收集的粉尘在溶血活性方面存在差异。